How does aquatic eDNA sampling work?
- chris10809
- Dec 30, 2024
- 2 min read
The process of eDNA analysis is remarkably sophisticated yet surprisingly straightforward:
Sample Collection: Water samples are carefully collected from a specific environment
DNA Extraction: Genetic material is isolated from the water
Amplification: Specific DNA segments are copied and multiplied
Analysis: Advanced genetic sequencing identifies species present
However, there are many nuances on how to sample that depends on the environment, ecology and research question/ aim of the eDNA sampling taking place (see Rees et al., 2014).
In an aquatic environment the current gold standard of eDNA sampling is through the filtration of water.
The sample water will contain a soup of DNA from the species that live in that environment (read more on what eDNA is here).
The DNA in the water can be free floating eDNA or DNA that is bound to a cell or particulates in the water (see Taberlet et al., 2018).
Using a specialized micro filter, the DNA is captured during filtration and can then be stored using a buffer or by freezing the filter to prevent the DNA from breaking down.
This breakthrough methodology allows researchers to unlock a comprehensive genetic snapshot of an entire aquatic environment without invasive sampling techniques.
Trace's Sampling Kits
Our kits have been meticulously designed with input from leading aquatic ecological researcher Dr Lewis Campbell.
Unlike traditional monitoring methods, our approach provides a non-invasive, highly accurate, and environmentally friendly solution to understanding the intricate web of life within water bodies – from microscopic bacteria to elusive vertebrate species.
With 100% biodegradable materials and carbon-neutral manufacturing, we're not just collecting data; we're pioneering a sustainable approach to ecological research that respects and protects the environments we study.
eDNA in Aquatic Environments: A Game-Changer for Ecological Research
Aquatic ecosystems present unique challenges for traditional monitoring methods. Fish are elusive, bacteria are invisible, and comprehensive surveys are time-consuming and expensive. eDNA changes everything.
Bacterial Monitoring: Unveiling Microscopic Communities
In water bodies, eDNA provides extraordinary insights into bacterial populations:
Detect potential pathogens
Monitor water quality
Understand microbial ecosystem dynamics
Track changes in bacterial communities over time
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